Generator

Part:BBa_I746903:Design

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-10-01)


T7 promoter driving 6-his tagged mut3 GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


Design Notes

Expression requires the presence of T7 DNA polymerase.


Source

The part was assembled from intermediates via standard assembly.

References